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酵母双杂交系统EasyClone cDNA Library Construction Kit
北京东方安诺生化科技有限公司
EasyClone
cDNA Library Construction Kit
Fast and Easy Synthesis of Highly Complex cDNAs
Directional cloning of cDNAs
The EasyClone cDNA Llibrary Construction Kit is designed to synthesize full-length-enriched
double stranded (ds) cDNA from total or polyA+ RNA, followed by subcloning into an appropriate library vector and amplification of the resulting cDNA library. The kit is based on a novel technology utilizing the specific features of MMLV-based reverse transcriptase to create full length enriched double stranded cDNA from total or polyA+ RNA samples. cDNAs are amplified by representational PCR and cloned directionally library vectors for use with for use with yeast two-hybrid systems such as Dualsystems' DUALmembrane and DUALhybrid protein interaction screening kits.
No extensive know-how in library construction is needed - the kit supplies all reagents needed for library construction and the detailed manual will guide you through all steps of construction.
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LITERATURE
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| User Manual |
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RELATED PRODUCTS
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| EasyClone Normalized cDNA Library Construction Kit |
| EasyClone PCR Kit |
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Manufactured by  |
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KIT CONTENTS - Box 1
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| 5X first strand buffer | 80 µl |
| DTT (20 mM) | 30 µl |
| 10X dNTP mix (10 mM each) | 25 µl |
| PlugOligo-1 adapter (15 µM) | 25 µl |
| CDS-1 adapter (10 µM) | 25 µl |
| PlugOligo-3M adapter (15 µM) | 25 µl |
| CDS-3M adapter (10 µM) | 25 µl |
| EasyClone reverse transcriptase | 20 µl |
| IP-solution | 130 µl |
| Control RNA template (0.5 µg/µl) | 15 µl |
| Sterile RNase free water | 1.8 ml |
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KIT CONTENTS - Box 2
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| 50X EasyClone polymerase mix | 50 µl |
| 10X EasyClone buffer | 300 µl |
| PCR Primer M1 (10 µM) | 100 µl |
| 50X dNTP mix (10 mM each) | 80 µl |
| Sterile RNase free water | 1.8 ml |
| Control amplified cDNA sample 1 | 25 µl |
| Control amplified cDNA sample 2 | 25 µl |
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